|Application ||WB, IHC-P, E|
|Reactivity||Human, Rabbit, Monkey|
|Dilution||ELISA (1:32000), IHC-P (5 µg/ml), WB (0.05-0.2 µg/ml)|
|Other Names||RUN and FYVE domain-containing protein 1, FYVE-finger protein EIP1, La-binding protein 1, Rab4-interacting protein, Zinc finger FYVE domain-containing protein 12, RUFY1, RABIP4, ZFYVE12|
|Target/Specificity||Human RUFY1. This antibody is expected to recognize both reported isoforms (NP_079434.3; NP_001035541.1). Reported variants represent identical protein: NP_001035542.1, NP_001035541.1.|
|Reconstitution & Storage||Store at -20°C. Minimize freezing and thawing.|
|Precautions||RUFY1 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.|
|Cellular Location||Cytoplasm. Early endosome membrane; Peripheral membrane protein|
|Tissue Location||Broadly expressed, with highest levels in lung, testis, kidney and brain.|
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Provided below are standard protocols that you may find useful for product applications.
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.
Fouraux M.A.,et al.Mol. Biol. Cell 15:611-624(2004).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Yang J.,et al.J. Biol. Chem. 277:30219-30226(2002).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
He J.,et al.Proteins 76:852-860(2009).
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