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QTRT1 Antibody

Rabbit Polyclonal Antibody

     
  • IHC - QTRT1 Antibody ALS16139
    Anti-QTRT1 antibody IHC staining of human kidney.
  • WB - QTRT1 Antibody ALS16139
    Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 10% SDS PAGE QTRT1 antibody diluted at 1:1000
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q9BXR0
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 44kDa
Dilution IHC-P (5 µg/ml), WB (1:500-1:3000),
Additional Information
Gene ID 81890
Other Names Queuine tRNA-ribosyltransferase, 2.4.2.29, Guanine insertion enzyme, tRNA-guanine transglycosylase, QTRT1, TGT, TGUT
Target/Specificity Human, mouse QTRT1
Reconstitution & Storage Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
PrecautionsQTRT1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name QTRT1 {ECO:0000255|HAMAP-Rule:MF_03218}
Synonyms TGT, TGUT
Function Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed:11255023, PubMed:20354154). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity).
Cellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03218}. Mitochondrion outer membrane {ECO:0000255|HAMAP-Rule:MF_03218}; Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_03218}; Cytoplasmic side {ECO:0000255|HAMAP-Rule:MF_03218}. Note=Weakly associates with mitochondria, possibly via QTRT2 {ECO:0000255|HAMAP-Rule:MF_03218}
Citations (0)

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Background

Interacts with QTRTD1 to form an active queuine tRNA- ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1- yl)amino)methyl)-7-deazaguanosine) (By similarity).

References

Deshpande K.L.,et al.Gene 265:205-212(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Grimwood J.,et al.Nature 428:529-535(2004).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).
Van Damme P.,et al.Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).

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$ 395.00
Cat# ALS16139
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