|Application ||WB, IHC-P, IF, ICC, E, Flo|
|Dilution||ELISA (1:10000), Flo (1:200-1:400), ICC (1:200-1:1000), IHC-P (10 µg/ml), WB (1:500-1:2000)|
|Other Names||Negative elongation factor A, NELF-A, Wolf-Hirschhorn syndrome candidate 2 protein, NELFA, WHSC2|
|Reconstitution & Storage||Short term +4°C; Long term -20°C|
|Precautions||NELFA / WHSC2 Antibody (aa280-511, clone 6B11H8) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex.|
|Tissue Location||Ubiquitous. Expressed in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. Expressed at lower level in adult lung. Expressed in fetal brain, lung, liver and kidney.|
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Provided below are standard protocols that you may find useful for product applications.
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex.
Wright T.J.,et al.Genomics 59:203-212(1999).
Ito M.,et al.Cancer Res. 61:2038-2046(2001).
Mei G.,et al.Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
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