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>   home   >   Products   >   Primary Antibodies   >   NELFA / WHSC2 Antibody (aa280-511, clone 6B11H8)   

NELFA / WHSC2 Antibody (aa280-511, clone 6B11H8)

Mouse Monoclonal Antibody

     
  • IHC - NELFA / WHSC2 Antibody (aa280-511, clone 6B11H8) ALS16190
    Anti-NELFA / WHSC2 antibody IHC staining of human prostate.
  • WB - NELFA / WHSC2 Antibody (aa280-511, clone 6B11H8) ALS16190
    Western blot using WHSC2 monoclonal antibody against HEK293 (1) and WHSC2 (AA: 280-511)-hIgGFc...
  • IF - NELFA / WHSC2 Antibody (aa280-511, clone 6B11H8) ALS16190
    Immunofluorescence of HeLa cells using WHSC2 mouse monoclonal antibody (green).
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, ICC, E, Flo
Primary Accession Q9H3P2
Reactivity Human, Rat
Host Mouse
Clonality Monoclonal
Clone Names 6B11H8
Calculated MW 57kDa
Dilution ELISA (1:10000), Flo (1:200-1:400), ICC (1:200-1:1000), IHC-P (10 µg/ml), WB (1:500-1:2000)
Additional Information
Gene ID 7469
Other Names Negative elongation factor A, NELF-A, Wolf-Hirschhorn syndrome candidate 2 protein, NELFA, WHSC2
Target/Specificity Human WHSC2
Reconstitution & Storage Short term +4°C; Long term -20°C
PrecautionsNELFA / WHSC2 Antibody (aa280-511, clone 6B11H8) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NELFA
Synonyms WHSC2
Function Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex.
Cellular Location Nucleus.
Tissue Location Ubiquitous. Expressed in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. Expressed at lower level in adult lung. Expressed in fetal brain, lung, liver and kidney.
Citations (0)

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Background

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex.

References

Wright T.J.,et al.Genomics 59:203-212(1999).
Ito M.,et al.Cancer Res. 61:2038-2046(2001).
Mei G.,et al.Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).

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$ 395.00
Cat# ALS16190
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