|Application ||WB, IHC-P|
|Reactivity||Human, Mouse, Rat|
|Dilution||IHC-P (7.5 µg/ml), WB (1:500-1:3000),|
|Other Names||Glutathione S-transferase theta-1, 220.127.116.11, GST class-theta-1, Glutathione transferase T1-1, GSTT1|
|Reconstitution & Storage||Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.|
|Precautions||GSTT1 Antibody (aa1-240) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2- epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4- nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide.|
|Tissue Location||Found in erythrocyte. Expressed at low levels in liver. In lung, expressed at low levels in Clara cells and ciliated cells at the alveolar/bronchiolar junction. Absent from epithelial cells of larger bronchioles|
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Provided below are standard protocols that you may find useful for product applications.
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2- epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4- nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide.
Pemble S.,et al.Biochem. J. 300:271-276(1994).
Jemth P.,et al.Arch. Biochem. Biophys. 348:247-254(1997).
Sprenger R.,et al.Pharmacogenetics 10:557-565(2000).
Iida A.,et al.Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004).
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