|Application ||WB, IHC-P|
|Dilution||IHC-P (7.5 µg/ml), WB (1:500-1:20000),|
|Other Names||E3 ubiquitin-protein ligase TRIM32, 6.3.2.-, 72 kDa Tat-interacting protein, Tripartite motif-containing protein 32, Zinc finger protein HT2A, TRIM32, HT2A|
|Target/Specificity||Human HT2A / TRIM32|
|Reconstitution & Storage||Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.|
|Precautions||HT2A / TRIM32 Antibody (aa1-289) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May ubiquitinate BBS2. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo.|
|Cellular Location||Cytoplasm. Note=Localized in cytoplasmic bodies, often located around the nucleus|
|Tissue Location||Spleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle|
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Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May ubiquitinate BBS2. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo.
Fridell R.A.,et al.Virology 209:347-357(1995).
Humphray S.J.,et al.Nature 429:369-374(2004).
Bienvenut W.V.,et al.Submitted (MAR-2009) to UniProtKB.
Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Locke M.,et al.Hum. Mol. Genet. 18:2344-2358(2009).
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