|Application ||WB, IHC-P|
|Dilution||IHC-P (7.5 µg/ml), WB (1:500-1:20000),|
|Other Names||E3 ubiquitin-protein ligase TRIM32, 6.3.2.-, 72 kDa Tat-interacting protein, Tripartite motif-containing protein 32, Zinc finger protein HT2A, TRIM32, HT2A|
|Target/Specificity||Human HT2A / TRIM32|
|Reconstitution & Storage||Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.|
|Precautions||HT2A / TRIM32 Antibody (aa1-289) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May ubiquitinate BBS2. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo.|
|Cellular Location||Cytoplasm. Note=Localized in cytoplasmic bodies, often located around the nucleus|
|Tissue Location||Spleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle|
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Provided below are standard protocols that you may find useful for product applications.
Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May ubiquitinate BBS2. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo.
Fridell R.A.,et al.Virology 209:347-357(1995).
Humphray S.J.,et al.Nature 429:369-374(2004).
Bienvenut W.V.,et al.Submitted (MAR-2009) to UniProtKB.
Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Locke M.,et al.Hum. Mol. Genet. 18:2344-2358(2009).
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