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HT2A / TRIM32 Antibody (aa1-289)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P |
|---|---|
| Primary Accession | Q13049 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 72kDa |
| Dilution | IHC-P (7.5 µg/ml), WB (1:500-1:20000), |
| Gene ID | 22954 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase TRIM32, 6.3.2.-, 72 kDa Tat-interacting protein, Tripartite motif-containing protein 32, Zinc finger protein HT2A, TRIM32, HT2A |
| Target/Specificity | Human HT2A / TRIM32 |
| Reconstitution & Storage | Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Precautions | HT2A / TRIM32 Antibody (aa1-289) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TRIM32 |
|---|---|
| Synonyms | HT2A |
| Function | E3 ubiquitin ligase that plays a role in various biological processes including neural stem cell differentiation, innate immunity, inflammatory resonse and autophagy (PubMed:19349376, PubMed:31123703). Plays a role in virus-triggered induction of IFN-beta and TNF-alpha by mediating the ubiquitination of STING1. Mechanistically, targets STING1 for 'Lys-63'-linked ubiquitination which promotes the interaction of STING1 with TBK1 (PubMed:22745133). Regulates bacterial clearance and promotes autophagy in Mycobacterium tuberculosis-infected macrophages (PubMed:37543647). Negatively regulates TLR3/4-mediated innate immune and inflammatory response by triggering the autophagic degradation of TICAM1 in an E3 activity-independent manner (PubMed:28898289). Plays an essential role in oxidative stress induced cell death by inducing loss of transmembrane potential and enhancing mitochondrial reactive oxygen species (ROS) production during oxidative stress conditions (PubMed:32918979). Ubiquitinates XIAP and targets it for proteasomal degradation (PubMed:21628460). Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation (PubMed:19349376). May ubiquitinate BBS2 (PubMed:22500027). Ubiquitinates PIAS4/PIASY and promotes its degradation in keratinocytes treated with UVB and TNF-alpha (By similarity). Also acts as a regulator of autophagy by mediating formation of unanchored 'Lys-63'-linked polyubiquitin chains that activate ULK1: interaction with AMBRA1 is required for ULK1 activation (PubMed:31123703). Positively regulates dendritic branching by promoting ubiquitination and subsequent degradation of the epigenetic factor CDYL (PubMed:34888944). |
| Cellular Location | Cytoplasm. Mitochondrion. Endoplasmic reticulum. Note=Localized in cytoplasmic bodies, often located around the nucleus |
| Tissue Location | Spleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle. |
| Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May ubiquitinate BBS2. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo.
References
Fridell R.A.,et al.Virology 209:347-357(1995).
Humphray S.J.,et al.Nature 429:369-374(2004).
Bienvenut W.V.,et al.Submitted (MAR-2009) to UniProtKB.
Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Locke M.,et al.Hum. Mol. Genet. 18:2344-2358(2009).
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