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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   NEDD8 Antibody (clone 5B8)   

NEDD8 Antibody (clone 5B8)

Mouse Monoclonal Antibody

     
  • IHC - NEDD8 Antibody (clone 5B8) ALS16266
    Anti-NEDD8 antibody IHC staining of human brain, cerebellum.
  • WB - NEDD8 Antibody (clone 5B8) ALS16266
    Western blot using NEDD8 mouse monoclonal antibody against C6 (1) and HeLa (2) cell lysate.
  • IF - NEDD8 Antibody (clone 5B8) ALS16266
    Immunofluorescence of HeLa cells using NEDD8 mouse monoclonal antibody (green).
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, ICC, E, Flo
Primary Accession Q15843
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5B8
Calculated MW 9kDa
Dilution ELISA (1:10000), Flo (1:200-1:400), ICC (1:200-1:1000), IF (1:1000), IHC-P (10 µg/ml), WB (1:500-1:2000),
Additional Information
Gene ID 4738
Other Names NEDD8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, NEDD-8, Ubiquitin-like protein Nedd8, NEDD8
Target/Specificity Human NEDD8
Reconstitution & Storage Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
PrecautionsNEDD8 Antibody (clone 5B8) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NEDD8
Function Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
Cellular Location Nucleus. Note=Mainly nuclear
Tissue Location Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes
Volume 50 µl
Research Areas
Citations (0)

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Background

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.

References

Osaka F.,et al.Genes Dev. 12:2263-2268(1998).
Ebert L.,et al.Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
Liakopoulos D.,et al.Proc. Natl. Acad. Sci. U.S.A. 96:5510-5515(1999).
Kamitani T.,et al.J. Biol. Chem. 272:28557-28562(1997).
Wada H.,et al.Biochem. Biophys. Res. Commun. 251:688-692(1998).

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$ 395.00
Cat# ALS16266
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