|Application ||WB, IHC-P, E|
|Reactivity||Human, Hamster, Horse, Bovine, Guinea Pig, Dog|
|Dilution||ELISA (1:128000), IHC-P (5 µg/ml), WB (0.1-0.3 µg/ml)|
|Other Names||Histamine H3 receptor, H3R, HH3R, G-protein coupled receptor 97, HRH3, GPCR97|
|Target/Specificity||Human HRH3 / Histamine 3 Receptor|
|Reconstitution & Storage||Store at -20°C. Minimize freezing and thawing.|
|Precautions||HRH3 / Histamine 3 Receptor Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of isoform 3 neither modified adenylate cyclase activity nor induced intracellular calcium mobilization.|
|Cellular Location||Cell membrane; Multi-pass membrane protein.|
|Tissue Location||Expressed predominantly in the CNS, with the greatest expression in the thalamus and caudate nucleus. The various isoforms are mainly coexpressed in brain, but their relative expression level varies in a region-specific manner Isoform 3 and isoform 7 are highly expressed in the thalamus, caudate nucleus and cerebellum while isoform 5 and isoform 6 show a poor expression. Isoform 5 and isoform 6 show a high expression in the amygdala, substantia nigra, cerebral cortex and hypothalamus. Isoform 7 is not found in hypothalamus or substantia nigra|
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Provided below are standard protocols that you may find useful for product applications.
The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of isoform 3 neither modified adenylate cyclase activity nor induced intracellular calcium mobilization.
Lovenberg T.W.,et al.Mol. Pharmacol. 55:1101-1107(1999).
Nakamura T.,et al.Biochem. Biophys. Res. Commun. 279:615-620(2000).
Coge F.,et al.Biochem. J. 355:279-288(2001).
Wiedemann P.,et al.J. Neural Transm. 109:443-453(2002).
Ullmer C.,et al.Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
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