|Application ||WB, IHC-P|
|Reactivity||Human, Mouse, Rat, Rabbit, Horse, Bovine|
|Dilution||IHC-P (5 µg/ml), WB (1:2500-1:10000),|
|Other Names||LIM domain and actin-binding protein 1, Epithelial protein lost in neoplasm, LIMA1, EPLIN, SREBP3|
|Target/Specificity||Region between residues 700 and the C-terminus (residue 759) of human epithelial protein lost in neoplasm beta using the numbering given in entry NP_057441.1 (GeneID 51474).|
|Reconstitution & Storage||Store at 2-8°C for up to 1 year.|
|Precautions||EPLIN Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments.|
|Cellular Location||Cytoplasm. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Note=This cytoskeletal protein co- localizes with actin stress fibers and focal adhesion plaques|
|Tissue Location||Highly expressed in placenta, kidney, pancreas, prostate, ovary, spleen and heart. Also detected in lung, liver, brain, skeletal muscle, thymus, testis and intestine Not detected in leukocytes. Isoform Beta expressed generally at very low levels. Isoform Alpha abundant in epithelial cells from mammary gland, prostate and in normal oral keratinocytes. Low levels in aortic endothelial cells and dermal fibroblasts. Not detectable in myocardium|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments.
Maul R.S.,et al.Oncogene 18:7838-7841(1999).
Hu R.-M.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
If you have any additional inquiries please email technical services at email@example.com.