|Application ||WB, IHC-P, IF, ICC|
|Reactivity||Human, Mouse, Rat, Rabbit, Pig, Bovine, Dog|
|Dilution||ICC (1:500-1:2000), IF (1:500-1:2000), IHC-P (5 µg/ml), WB (1:1000-1:10000) ,|
|Other Names||Cohesin subunit SA-2, SCC3 homolog 2, Stromal antigen 2, STAG2, SA2|
|Target/Specificity||Region between residues 1100 and 1150 of human Stromal Antigen 2 using the numbering given in entry NP_006594.3 (GeneID 10735).|
|Reconstitution & Storage||Store at 2-8°C for up to 1 year.|
|Precautions||STAG2 Antibody (aa1100-1150) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.|
|Cellular Location||Nucleus. Chromosome. Chromosome, centromere. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK1, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex|
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Provided below are standard protocols that you may find useful for product applications.
Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Ross M.T.,et al.Nature 434:325-337(2005).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Carramolino L.,et al.Gene 195:151-159(1997).
Carramolino L.,et al.Gene 206:283-286(1998).
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