|Application ||IHC-P, IF, IP|
|Reactivity||Human, Rabbit, Horse|
|Dilution||IF (1:250-1:1000), IHC-P (5 µg/ml), IP (2-5 µg/mg lysate)|
|Other Names||Kinesin-like protein KIF2C, Kinesin-like protein 6, Mitotic centromere-associated kinesin, MCAK, KIF2C, KNSL6|
|Target/Specificity||Region between residue 50 and 100 of human Mitotic Centromere-Associated Kinesin using the numbering given in entry NP_006836.1 (GeneID 11004).|
|Reconstitution & Storage||Store at 2-8°C for up to 1 year.|
|Precautions||KIF2C / MCAK Antibody (aa50-100) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis.|
|Cellular Location||Cytoplasm, cytoskeleton. Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore. Note=Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Association with microtubule plus ends is also mediated by interaction with KIF18B. Centromeric localization requires the presence of BUB1 and SGOL2.|
|Tissue Location||Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific.|
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Provided below are standard protocols that you may find useful for product applications.
In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis.
Kim I.-G.,et al.Biochim. Biophys. Acta 1359:181-186(1997).
Cheng L.J.,et al.Life Sci. 71:2741-2757(2002).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
Katagiri T.,et al.Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
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