|Application ||WB, IHC-P, ICC|
|Reactivity||Human, Mouse, Rat|
|Dilution||ICC (1 µg/ml), IHC-P (5 µg/ml), WB (1 µg/ml)|
|Other Names||CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD154, CD40 ligand, membrane form, CD40 ligand, soluble form, CD40LG, CD40L, TNFSF5, TRAP|
|Target/Specificity||Human, mouse, rat. No cross reactivity with other proteins.|
|Reconstitution & Storage||Short term +4°C; Long term -20°C|
|Precautions||CD40L Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CD40L, TNFSF5, TRAP|
|Function||Cytokine that binds to CD40/TNFRSF5 (PubMed:1280226). Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T- cells. Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching (By similarity).|
|Cellular Location||Cell membrane; Single-pass type II membrane protein Cell surface|
|Tissue Location||Specifically expressed on activated CD4+ T- lymphocytes|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Graf D.,et al.Eur. J. Immunol. 22:3191-3194(1992).
Hollenbaugh D.,et al.EMBO J. 11:4313-4321(1992).
Aruffo A.,et al.Cell 72:291-300(1993).
Spriggs M.K.,et al.J. Exp. Med. 176:1543-1550(1992).
Gauchat J.-F.,et al.FEBS Lett. 315:259-266(1993).
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