|Application ||WB, IHC-P, ICC|
|Reactivity||Human, Mouse, Rat|
|Dilution||ICC (1 µg/ml), IHC-P (5 µg/ml), WB (1 µg/ml)|
|Other Names||CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD154, CD40 ligand, membrane form, CD40 ligand, soluble form, CD40LG, CD40L, TNFSF5, TRAP|
|Target/Specificity||Human, mouse, rat. No cross reactivity with other proteins.|
|Reconstitution & Storage||Short term +4°C; Long term -20°C|
|Precautions||CD40L Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CD40L, TNFSF5, TRAP|
|Function||Cytokine that binds to CD40/TNFRSF5 (PubMed:1280226). Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T- cells. Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching (By similarity).|
|Cellular Location||Cell membrane; Single-pass type II membrane protein Cell surface|
|Tissue Location||Specifically expressed on activated CD4+ T- lymphocytes|
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Provided below are standard protocols that you may find useful for product applications.
Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Graf D.,et al.Eur. J. Immunol. 22:3191-3194(1992).
Hollenbaugh D.,et al.EMBO J. 11:4313-4321(1992).
Aruffo A.,et al.Cell 72:291-300(1993).
Spriggs M.K.,et al.J. Exp. Med. 176:1543-1550(1992).
Gauchat J.-F.,et al.FEBS Lett. 315:259-266(1993).
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