APPL1 / APPL Antibody (aa111-160)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | Q9UKG1 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 80kDa |
Dilution | ELISA (1:10000), IHC-P (20 µg/ml), WB (1:500-1:1000) |
Gene ID | 26060 |
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Other Names | DCC-interacting protein 13-alpha, Dip13-alpha, Adapter protein containing PH domain, PTB domain and leucine zipper motif 1, APPL1 |
Target/Specificity | APPL1 Antibodyantibody detects endogenous levels of APPL1. |
Reconstitution & Storage | Store at -20°C. |
Precautions | APPL1 / APPL Antibody (aa111-160) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | APPL1 (HGNC:24035) |
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Function | Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism (PubMed:26583432, PubMed:15016378, PubMed:26073777, PubMed:19661063, PubMed:10490823). Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and subunits of the NuRD/MeCP1 complex (PubMed:15016378). Functions as a positive regulator of innate immune response via activation of AKT1 signaling pathway by forming a complex with APPL1 and PIK3R1 (By similarity). Inhibits Fc-gamma receptor-mediated phagocytosis through PI3K/Akt signaling in macrophages (By similarity). Regulates TLR4 signaling in activated macrophages (By similarity). Involved in trafficking of the TGFBR1 from the endosomes to the nucleus via microtubules in a TRAF6-dependent manner (PubMed:26583432). Plays a role in cell metabolism by regulating adiponecting and insulin signaling pathways (PubMed:26073777, PubMed:19661063, PubMed:24879834). Required for fibroblast migration through HGF cell signaling (By similarity). Positive regulator of beta-catenin/TCF-dependent transcription through direct interaction with RUVBL2/reptin resulting in the relief of RUVBL2-mediated repression of beta-catenin/TCF target genes by modulating the interactions within the beta-catenin-reptin- HDAC complex (PubMed:19433865). |
Cellular Location | Early endosome membrane; Peripheral membrane protein. Nucleus. Cytoplasm. Endosome. Cell projection, ruffle {ECO:0000250|UniProtKB:Q8K3H0}. Cytoplasmic vesicle, phagosome {ECO:0000250|UniProtKB:Q8K3H0}. Note=Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF |
Tissue Location | High levels in heart, ovary, pancreas and skeletal muscle. |
Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction.
References
Mitsuuchi Y.,et al.Oncogene 18:4891-4898(1999).
Liu J.,et al.J. Biol. Chem. 277:26281-26285(2002).
Nagase T.,et al.DNA Res. 7:65-73(2000).
Nakajima D.,et al.DNA Res. 9:99-106(2002).
Miaczynska M.,et al.Cell 116:445-456(2004).
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