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CDC42BPB / MRCKB Antibody (Phe1665)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P |
|---|---|
| Primary Accession | Q9Y5S2 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 194kDa |
| Dilution | IHC-P (5 µg/ml), WB (1:500-1:1000), |
| Gene ID | 9578 |
|---|---|
| Other Names | Serine/threonine-protein kinase MRCK beta, 2.7.11.1, CDC42-binding protein kinase beta, CDC42BP-beta, DMPK-like beta, Myotonic dystrophy kinase-related CDC42-binding kinase beta, MRCK beta, Myotonic dystrophy protein kinase-like beta, CDC42BPB {ECO:0000312|EMBL:AAD37506.1} |
| Target/Specificity | Human CDC42BPB |
| Reconstitution & Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Precautions | CDC42BPB / MRCKB Antibody (Phe1665) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CDC42BPB {ECO:0000312|EMBL:AAD37506.1} |
|---|---|
| Function | Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2 (PubMed:21457715, PubMed:21949762). In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (PubMed:18854160). Phosphorylates PPP1R12A (PubMed:21457715). In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (By similarity). |
| Cellular Location | Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction. Cell projection, lamellipodium {ECO:0000250|UniProtKB:Q3UU96}. Note=Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH- domain dependent (By similarity). Detected at the leading edge of migrating cells. Localization at the leading edge of migrating cells requires interaction with catalytically active CDC42 (PubMed:21240187) Localizes in the lamellipodium in a FAM89B/LRAP25-dependent manner (By similarity). {ECO:0000250|UniProtKB:O54874, ECO:0000250|UniProtKB:Q3UU96, ECO:0000269|PubMed:21240187} |
| Tissue Location | Expressed in all tissues examined, with high levels in heart, brain, placenta and lung. |
| Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A.
References
Moncrieff C.L.,et al.Genomics 57:297-300(1999).
Hirosawa M.,et al.DNA Res. 6:329-336(1999).
Nakajima D.,et al.DNA Res. 9:99-106(2002).
Olsen J.V.,et al.Cell 127:635-648(2006).
Tan I.,et al.Cell 135:123-136(2008).
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