|Application ||WB, IHC-P, IF|
|Reactivity||Human, Mouse, Rat, Monkey, Dog|
|Dilution||IF (1:50-1:200), IHC-P (5 µg/ml), WB (1:250-1:1000)|
|Other Names||Ras-related protein Rab-27A, Rab-27, GTP-binding protein Ram, RAB27A, RAB27|
|Target/Specificity||Detects Rab27a by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab27a. This antibody is specific for Rab27a. It does not recognise Rab27b protein.|
|Reconstitution & Storage||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
|Precautions||RAB27A / RAB27 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.|
|Cellular Location||Membrane; Lipid-anchor. Melanosome. Late endosome. Lysosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545, PubMed:17081065). Localizes to endosomal exocytic vesicles (PubMed:17237785).|
|Tissue Location||Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.|
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Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
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