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RAB5A / RAB5 Antibody (C-Terminus)
Goat Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF |
|---|---|
| Primary Accession | P20339 |
| Reactivity | Human, Mouse, Rat, Monkey, Dog |
| Host | Goat |
| Clonality | Polyclonal |
| Calculated MW | 24kDa |
| Dilution | IF (1:50-1:250), IHC-P (5 µg/ml), WB (1:250-1:2000) |
| Gene ID | 5868 |
|---|---|
| Other Names | Ras-related protein Rab-5A, RAB5A, RAB5 |
| Target/Specificity | Detects total Rab5 protein in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab5a, GFP-Rab5b and GFP-Rab5c cds by Western blot. |
| Reconstitution & Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Precautions | RAB5A / RAB5 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RAB5A |
|---|---|
| Synonyms | RAB5 |
| Function | Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity). |
| Cellular Location | Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid- anchor. Melanosome. Cytoplasmic vesicle. Cell projection, ruffle {ECO:0000250|UniProtKB:P18066}. Membrane Cytoplasm, cytosol. Cytoplasmic vesicle, phagosome membrane {ECO:0000250|UniProtKB:Q9CQD1}. Endosome membrane Note=Enriched in stage I melanosomes (PubMed:17081065). Alternates between membrane-bound and cytosolic forms (Probable) {ECO:0000269|PubMed:17081065, ECO:0000305} |
| Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension.
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