|Application ||WB, IHC-P|
|Reactivity||Human, Mouse, Rat, Pig, Sheep|
|Calculated MW||56795 Da|
|Dilution||IHC-P (5 µg/ml),|
|Other Names||LIPG, EDL, EL, Lipoprotein lipase H, Lipase, endothelial, PRO719, Endothelial lipase|
|Target/Specificity||human endothelial lipase amino acids 19-32|
|Reconstitution & Storage||TBS, pH 7.4, 0.02% sodium azide, 0.5 mg/ml BSA, 50% glycerol. Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||LIPG / Endothelial Lipase Antibody (aa19-32) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin.|
|Tissue Location||High level of expression in the liver, placenta, lung, thyroid, kidney, testis and in the corpus luteum of the ovary. Expressed also in coronary artery endothelial cells, umbilical vein endothelial cells and in hepatocytes and osteosarcoma cell lines. Not detected in heart, brain and muscle|
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Provided below are standard protocols that you may find useful for product applications.
Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin.
Hirata K.,et al.J. Biol. Chem. 274:14170-14175(1999).
Jaye M.,et al.Nat. Genet. 21:424-428(1999).
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
McCoy M.G.,et al.J. Lipid Res. 43:921-929(2002).
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