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Vimentin Antibody (clone V9)

Mouse Monoclonal Antibody

     
  • IHC - Vimentin Antibody (clone V9) ALS16522
    Anti-Vimentin antibody IHC of human prostate.
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  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, ICC, IHC-Fr, Flo
Primary Accession P08670
Other Accession 7431
Reactivity Human, Rat, Chicken, Sheep
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names V9
Calculated MW 53652 Da
Dilution Flo (1:25 - 1:200), IHC-Fr (1:25 - 1:200), IHC-P (20 µg/ml), WB (1:100 - 1:1000),
Additional Information
Gene ID 7431
Other Names VIM, Vimentin
Target/Specificity Reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
Reconstitution & Storage PBS containing 0.09% sodium azide. Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
PrecautionsVimentin Antibody (clone V9) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VIM
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix {ECO:0000250|UniProtKB:P31000}. Cell membrane {ECO:0000250|UniProtKB:P20152}
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Volume 50 µl
Research Areas
Citations (0)
citation

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Background

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

References

Ferrari S.,et al.Mol. Cell. Biol. 6:3614-3620(1986).
Honore B.,et al.Nucleic Acids Res. 18:6692-6692(1990).
Hartmann T.B.,et al.Br. J. Dermatol. 150:252-258(2004).
Zimbelmann R.,et al.Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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Discontinued
Cat# ALS16522
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