|Application ||WB, IHC-P, ICC, IHC-Fr, Flo|
|Reactivity||Human, Rat, Chicken, Sheep|
|Calculated MW||53652 Da|
|Dilution||Flo (1:25 - 1:200), IHC-Fr (1:25 - 1:200), IHC-P (20 µg/ml), WB (1:100 - 1:1000),|
|Other Names||VIM, Vimentin|
|Target/Specificity||Reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.|
|Reconstitution & Storage||PBS containing 0.09% sodium azide. Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.|
|Precautions||Vimentin Antibody (clone V9) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.|
|Tissue Location||Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines|
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Provided below are standard protocols that you may find useful for product applications.
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Ferrari S.,et al.Mol. Cell. Biol. 6:3614-3620(1986).
Honore B.,et al.Nucleic Acids Res. 18:6692-6692(1990).
Hartmann T.B.,et al.Br. J. Dermatol. 150:252-258(2004).
Zimbelmann R.,et al.Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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