Vimentin Antibody (clone V9)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, ICC, IHC-Fr, Flo |
---|---|
Primary Accession | P08670 |
Other Accession | 7431 |
Reactivity | Human, Rat, Chicken, Sheep |
Host | Mouse |
Clonality | Monoclonal |
Isotype | IgG1 |
Clone Names | V9 |
Calculated MW | 53652 Da |
Dilution | Flo (1:25 - 1:200), IHC-Fr (1:25 - 1:200), IHC-P (20 µg/ml), WB (1:100 - 1:1000), |
Gene ID | 7431 |
---|---|
Other Names | VIM, Vimentin |
Target/Specificity | Reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. |
Reconstitution & Storage | PBS containing 0.09% sodium azide. Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted. |
Precautions | Vimentin Antibody (clone V9) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | VIM |
---|---|
Function | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. |
Cellular Location | Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix {ECO:0000250|UniProtKB:P31000}. Cell membrane {ECO:0000250|UniProtKB:P20152} |
Tissue Location | Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. |
Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
References
Ferrari S.,et al.Mol. Cell. Biol. 6:3614-3620(1986).
Honore B.,et al.Nucleic Acids Res. 18:6692-6692(1990).
Hartmann T.B.,et al.Br. J. Dermatol. 150:252-258(2004).
Zimbelmann R.,et al.Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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