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ERBB2 / HER2 Antibody (clone 3B5)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IP, IHC-F |
|---|---|
| Primary Accession | P04626 |
| Other Accession | 2064 |
| Reactivity | Human, Mouse, Rat, Monkey |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone Names | 3B5 |
| Calculated MW | 137910 Da |
| Dilution | IHC-P (10 µg/ml), WB (1:250 - 1:1000), WB (1:250 - 1:1000), |
| Gene ID | 2064 |
|---|---|
| Other Names | ERBB2, C-erb B2/neu protein, CD340, HER2, Herstatin, MLN 19, HER-2, Neu, NGL, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, CD340 antigen, MLN19, p185erbB2, TKR1 |
| Target/Specificity | Sequence is identical in rat Neu protein. Reacts equally well with the wild as well as the mutant (oncogenic) form c-erbB-2 protein but preferentially recognizes the unphosphorylated form of c-erbB-2 protein. |
| Reconstitution & Storage | PBS containing 0.09% sodium azide. Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted. |
| Precautions | ERBB2 / HER2 Antibody (clone 3B5) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ERBB2 |
|---|---|
| Synonyms | HER2, MLN19, NEU, NGL |
| Function | Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. |
| Cellular Location | Cell membrane; Single-pass type I membrane protein. Cell projection, ruffle membrane; Single-pass type I membrane protein. Note=Internalized from the cell membrane in response to EGF stimulation. [Isoform 2]: Cytoplasm. Nucleus. |
| Tissue Location | Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
References
Yamamoto T.,et al.Nature 319:230-234(1986).
Coussens L.,et al.Science 230:1132-1139(1985).
Wakamatsu A.,et al.Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Tal M.,et al.Mol. Cell. Biol. 7:2597-2601(1987).
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