|Application ||IHC-P, IHC-Fr, Flo|
|Calculated MW||122102 Da|
|Dilution||IHC-P (10 µg/ml),|
|Other Names||MUC1, CA15-3, CD227, Carcinoma-associated mucin, CD227 antigen, DF3 antigen, Episialin, Epithelial membrane antigen, H23 antigen, H23AG, Krebs von den Lungen-6, MAM6, MUC1/ZD, MUC-1/SEC, Mucin-1, Pem, KL-6, MUC-1/X, Tumor-associated mucin, Peanut-rea ...|
|Target/Specificity||directed against the repeat domain of episialin.|
|Reconstitution & Storage||PBS containing 0.09% sodium azide. Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.|
|Precautions||EMA / MUC1 Antibody (clone 214D4) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.|
|Cellular Location||Apical cell membrane; Single- pass type I membrane protein. Note=Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane. Located to microvilli and to the tips of long filopodial protusions Isoform Y: Secreted. Mucin-1 subunit beta: Cell membrane. Cytoplasm. Nucleus. Note=On EGF and PDGFRB stimulation, transported to the nucleus through interaction with CTNNB1, a process which is stimulated by phosphorylation. On HRG stimulation, colocalizes with JUP/gamma-catenin at the nucleus|
|Tissue Location||Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform Y is expressed in tumor cells only.|
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Provided below are standard protocols that you may find useful for product applications.
The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
Lan M.S.,et al.J. Biol. Chem. 265:15294-15299(1990).
Ligtenberg M.J.L.,et al.J. Biol. Chem. 265:5573-5578(1990).
Gendler S.J.,et al.J. Biol. Chem. 265:15286-15293(1990).
Lancaster C.A.,et al.Biochem. Biophys. Res. Commun. 173:1019-1029(1990).
Wreschner D.H.,et al.Eur. J. Biochem. 189:463-473(1990).
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