|Application ||WB, IHC-P, IF, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||160970 Da|
|Dilution||ELISA (1:1000), IF (1:100 - 1:500), IHC-P (20 µg/ml), WB (1:500 - 1:1000),|
|Other Names||NOS1, BNOS, Constitutive NOS, IHPS1, NNOS, NC-NOS, Nitric oxide synthase, brain, N-NOS, Neuronal NOS, NOS, NOS type I, Nos, type i|
|Target/Specificity||nNOS Antibody detects endogenous levels of total nNOS protein.|
|Reconstitution & Storage||PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol. Store at -20°C for up to one year.|
|Precautions||NOS1 / nNOS Antibody (aa818-867) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.|
|Cellular Location||Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity).|
|Tissue Location||Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
Hall A.V.,et al.J. Biol. Chem. 269:33082-33090(1994).
Fujisawa H.,et al.J. Neurochem. 63:140-145(1994).
Nakane M.,et al.FEBS Lett. 316:175-180(1993).
Park C.-S.,et al.Cell. Mol. Neurobiol. 16:499-515(1996).
Wang Y.,et al.J. Biol. Chem. 272:11392-11401(1997).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.