CYP2B6 Antibody (aa241-290)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | P20813 |
Other Accession | 1555 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 56278 Da |
Dilution | ELISA (1:1000), IHC-P (20 µg/ml), WB (1:500 - 1:1000), |
Gene ID | 1555 |
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Other Names | CYP2B6, CPB6, CYP2B, CYP2B7P, EFVM, CYP2B7, Cytochrome p450 IIb6, p450, CYPIIB6, Cytochrome P450 2B6, Cytochrome P450 IIB1, IIB1 |
Target/Specificity | Cytochrome P450 2B6 Antibody detects endogenous levels of total Cytochrome P450 2B6 protein. |
Reconstitution & Storage | PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol. Store at -20°C for up to one year. |
Precautions | CYP2B6 Antibody (aa241-290) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CYP2B6 {ECO:0000303|PubMed:21289075, ECO:0000312|HGNC:HGNC:2615} |
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Function | A cytochrome P450 monooxygenase involved in the metabolism of endocannabinoids and steroids (PubMed:21289075, PubMed:12865317). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH-- hemoprotein reductase). Catalyzes the epoxidation of double bonds of arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15- epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling (PubMed:21289075). Hydroxylates steroid hormones, including testosterone at C-16 and estrogens at C-2 (PubMed:21289075, PubMed:12865317). Plays a role in the oxidative metabolism of xenobiotics, including plant lipids and drugs (PubMed:11695850, PubMed:22909231). Acts as a 1,4-cineole 2-exo- monooxygenase (PubMed:11695850). |
Cellular Location | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein |
Tissue Location | Expressed in liver, lung and heart right ventricle. |
Volume | 50 µl |
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Provided below are standard protocols that you may find useful for product applications.
Background
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,4-cineole 2-exo-monooxygenase.
References
Yamano S.,et al.Biochemistry 28:7340-7348(1989).
Zhuge J.,et al.Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Grimwood J.,et al.Nature 428:529-535(2004).
Miles J.S.,et al.Nucleic Acids Res. 17:8241-8255(1989).
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