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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   SET / TAF-I Antibody   

SET / TAF-I Antibody

Rabbit Polyclonal Antibody

     
  • IHC - SET / TAF-I Antibody ALS16587
    Anti-SET / TAF-I antibody IHC staining of human small intestine.
  • ICC - SET / TAF-I Antibody ALS16587
    Immunocytochemistry: Hela
  • WB - SET / TAF-I Antibody ALS16587
    western blotting
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, ICC
Primary Accession Q01105
Other Accession 6418
Reactivity Human, Mouse, Rat, Monkey, Chicken, Bovine
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 33489 Da
Dilution ICC (1:100), IHC-P (5 µg/ml), WB (1:2000),
Additional Information
Gene ID 6418
Other Names SET, 2PP2A, HLA-DR-associated protein II, I-2PP2A, TAF-IBETA, SET nuclear oncogene, TAF-I, Template-activating factor I, I2PP2A, IGAAD, IPP2A2, PHAPII, Protein SET
Target/Specificity Human SET
Reconstitution & Storage PBS, pH 7.2, 50% glycerol. Store at -20°C.
PrecautionsSET / TAF-I Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SET
Function Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T- lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
Cellular Location Cytoplasm, cytosol. Endoplasmic reticulum. Nucleus, nucleoplasm. Note=In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. The SET complex is associated with the endoplasmic reticulum. Following CTL attack and cleavage by GZMA, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium
Tissue Location Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor
Volume 500 µl
Research Areas
Citations (0)

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Background

Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T- lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.

References

von Lindern M.,et al.Mol. Cell. Biol. 12:3346-3355(1992).
Vaesen M.,et al.Biol. Chem. Hoppe-Seyler 375:113-126(1994).
Nagata K.,et al.Proc. Natl. Acad. Sci. U.S.A. 92:4279-4283(1995).
Li M.,et al.J. Biol. Chem. 271:11059-11062(1996).
Tsujio I.,et al.FEBS Lett. 579:363-372(2005).

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$ 395.00
Cat# ALS16587
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