|Application ||IHC-P, E|
|Reactivity||Human, Mouse, Rat, Hamster, Monkey, Pig, Chicken, Xenopus, Bovine, Dog|
|Calculated MW||19196 Da|
|Dilution||ELISA (1:16000), IHC-P (10 µg/ml),|
|Other Names||BOD1, Biorientation defective 1, Protein FAM44B, FAM44B|
|Target/Specificity||Human BOD1. This antibody is expected to recognize isoforms a and b (NP_612378.1; NP_001153123.1).|
|Reconstitution & Storage||Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide. Store at -20°C. Minimize freezing and thawing.|
|Precautions||BOD1 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for proper chromosome biorientation through the detection or correction of syntelic attachments in mitotic spindles.|
|Cellular Location||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Chromosome, centromere, kinetochore Note=Localizes at the centrosomes throughout the cell cycle, only dissociating during cytokinesis. Localizes at the kinetochore from prometaphase until anaphase|
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Provided below are standard protocols that you may find useful for product applications.
Required for proper chromosome biorientation through the detection or correction of syntelic attachments in mitotic spindles.
Li H.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Porter I.M.,et al.J. Cell Biol. 179:187-197(2007).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).
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