|Application ||IHC-P, E|
|Reactivity||Human, Mouse, Rat, Pig, Horse|
|Calculated MW||70818 Da|
|Dilution||ELISA (1:32000), IHC-P (5 µg/ml),|
|Other Names||SPAG16, Pf20 protein homolog, WDR29, PF20, Sperm associated antigen 16, WD repeat domain 29|
|Target/Specificity||Human SPAG16. This antibody is expected to recognize isoform 1 (NP_078808.3) only.|
|Reconstitution & Storage||Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide. Store at -20°C. Minimize freezing and thawing.|
|Precautions||SPAG16 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia (By similarity).|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton, flagellum axoneme. Cytoplasm, cytoskeleton, cilium axoneme. Note=Detected on the sperm flagellum axoneme. Detected in the central apparatus of the axoneme. Colocalizes with SPAG6 on microtubules (By similarity)|
|Tissue Location||Isoform 1 is detected in testis. Isoform 4 is detected in testis and brain, and at lower levels in kidney, heart, pancreas, thyroid, ovary, adrenal gland, spinal cord, trachea and liver.|
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Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia (By similarity).
Pennarun G.,et al.Am. J. Respir. Cell Mol. Biol. 26:362-370(2002).
Zhang Z.,et al.Mol. Cell. Biol. 22:7993-8004(2002).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
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