KIAA0692 / ANKLE2 Antibody (Internal)
Chicken Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
---|---|
Primary Accession | Q86XL3 |
Other Accession | 23141 |
Reactivity | Human, Mouse, Rat |
Host | Chicken |
Clonality | Polyclonal |
Isotype | IgY |
Calculated MW | 104114 Da |
Dilution | IHC-P (5 µg/ml), WB (1 - 2 µg/ml), |
Gene ID | 23141 |
---|---|
Other Names | ANKLE2, KIAA0692, LEMD7, LEM domain containing 7, Lem4 |
Target/Specificity | Human ANKLE2 / KIAA0692. Three isoforms of ANKLE2 are known to exist; this antibody will detect all three. ANKLE2 antibody is predicted to not cross-react with ANKLE2. |
Reconstitution & Storage | PBS, 0.02% sodium azide. Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted. |
Precautions | KIAA0692 / ANKLE2 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ANKLE2 |
---|---|
Synonyms | KIAA0692, LEM4 |
Function | Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit (PubMed:22770216). Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly (PubMed:22770216). May regulate nuclear localization of VRK1 in non- dividing cells (PubMed:31735666). It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex (PubMed:22770216). Involved in brain development (PubMed:25259927). |
Cellular Location | Endoplasmic reticulum membrane; Single-pass type III membrane protein |
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Background
Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit. Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly. It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex.
References
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Scherer S.E.,et al.Nature 440:346-351(2006).
Ishikawa K.,et al.DNA Res. 5:169-176(1998).
Cantin G.T.,et al.J. Proteome Res. 7:1346-1351(2008).
Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
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