|Application ||WB, IHC-P, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||43381 Da|
|Dilution||ELISA (1:40000), IHC-P (10 µg/ml), WB (1:500 - 1:1000),|
|Other Names||AGPAT3, 1-AGP acyltransferase 3, 1-AGPAT 3, LPAAT3, LPAAT-gamma, LPAAT-GAMMA1|
|Target/Specificity||AGPAT3 Antibody detects endogenous levels of total AGPAT3 protein.|
|Reconstitution & Storage||PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol. Store at -20°C for up to one year.|
|Precautions||AGPAT3 Antibody (aa121-170) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Acts on LPA containing saturated or unsaturated fatty acids C16:0-C20:4 at the sn-1 position using C18:1, C20:4 or C18:2-CoA as the acyl donor. Also acts on lysophosphatidylcholine, lysophosphatidylinositol and lysophosphatidylserine using C18:1 or C20:4-CoA (PubMed:21173190). Has a preference for arachidonoyl-CoA as a donor. Has also a modest lysophosphatidylinositol acyltransferase (LPIAT) activity, converts lysophosphatidylinositol (LPI) into phosphatidylinositol (By similarity).|
|Cellular Location||Endoplasmic reticulum membrane; Multi- pass membrane protein. Nucleus envelope|
|Tissue Location||Widely expressed with highest levels in testis, pancreas and kidney, followed by spleen, lung, adipose tissue and liver.|
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Provided below are standard protocols that you may find useful for product applications.
Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Acts on LPA containing saturated or unsaturated fatty acids C16:0-C20:4 at the sn-1 position using C18:1, C20:4 or C18:2-CoA as the acyl donor. Also acts on lysophosphatidylcholine, lysophosphatidylinositol and lysophosphatidylserine using C18:1 or C20:4-CoA.
Leung D.W.,et al.Front. Biosci. 6:D944-D953(2001).
Nagamine K.,et al.Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Hattori M.,et al.Nature 405:311-319(2000).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
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