|Application ||WB, IHC-P, IF|
|Reactivity||Human, Mouse, Rat, Monkey, Dog|
|Calculated MW||56782 Da|
|Dilution||IF (1:50 - 1:500), IHC-P (5 µg/ml), WB (1:500 - 1:2000),|
|Other Names||PDIA3, 58 kDa microsomal protein, ER protein 57, ERp60, ERp61, Disulfide isomerase ER-60, Endoplasmic reticulum P58, GRP58, HsT17083, p58, Phospholipase C-alpha, PI-PLC, ER protein 60, ER60, ERp57, GRP57, Protein disulfide-isomerase A3|
|Target/Specificity||Detects a band of 60 kDa by Western blot in the following canine, human, monkey, mouse, rat whole cell lysates.|
|Reconstitution & Storage||PBS, 20% glycerol, 0.05% sodium azide. Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
|Precautions||PDIA3 / ERp57 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ERP57, ERP60, GRP58|
|Cellular Location||Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545).|
|Tissue Location||Detected in the flagellum and head region of spermatozoa (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
Hirano N.,et al.Biochem. Biophys. Res. Commun. 204:375-382(1994).
Bourdi M.,et al.Arch. Biochem. Biophys. 323:397-403(1995).
Koivunen P.,et al.Biochem. J. 316:599-605(1996).
Charnock-Jones D.S.,et al.Int. J. Biochem. Cell Biol. 28:81-89(1996).
Koivunen P.,et al.Genomics 42:397-404(1997).
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