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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RAB8A / RAB8 Antibody (C-Terminus)   

RAB8A / RAB8 Antibody (C-Terminus)

Goat Polyclonal Antibody

     
  • IHC - RAB8A / RAB8 Antibody (C-Terminus) ALS16654
    Anti-RAB8A / RAB8 antibody IHC staining of human kidney.
  • IF - RAB8A / RAB8 Antibody (C-Terminus) ALS16654
    Immunofluorescence - anti-Rab8 antibody at 1:100 dilution using primary macrophages.
  • WB - RAB8A / RAB8 Antibody (C-Terminus) ALS16654
    Western blot.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF
Primary Accession P61006
Other Accession 4218
Reactivity Human, Mouse, Rat
Host Goat
Clonality Polyclonal
Isotype IgG
Calculated MW 23668 Da
Dilution IF (1:100 - 1:250), IHC-P (5 µg/ml), WB (1:250 - 1:2000),
Additional Information
Gene ID 4218
Other Names RAB8A, RAB8, Ras-related protein rab-8, Oncogene c-mel, Ras-associated protein RAB8, Ras-related protein Rab-8A, MEL
Target/Specificity Detects a band of 24 kDa by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab8a and GFP-Rab8b cds.
Reconstitution & Storage PBS, 20% glycerol, 0.05% sodium azide. Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
PrecautionsRAB8A / RAB8 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAB8A
Synonyms MEL, RAB8
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Plays an important role in ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly. May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis.
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus. Recycling endosome membrane. Cell projection, cilium. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle, phagosome membrane {ECO:0000250|UniProtKB:Q92930}; Lipid-anchor {ECO:0000250|UniProtKB:Q92930}; Cytoplasmic side {ECO:0000250|UniProtKB:Q92930}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250|UniProtKB:P55258}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:P55258}. Note=Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane (PubMed:15837803). In the GDP-bound form, present in the perinuclear region (PubMed:12221131). Shows a polarized distribution to distal regions of cell protrusions in the GTP- bound form (PubMed:12221131). Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis (PubMed:20890297). Localizes to tubular recycling endosome (PubMed:19864458). Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211).
Volume 50 µl
Research Areas
Citations (0)

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Background

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Plays an important role in ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly. May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis.

References

Zahraoui A.,et al.J. Cell Biol. 124:101-115(1994).
Nimmo E.R.,et al.Oncogene 6:1347-1351(1991).
Puhl H.L. III,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 395.00
Cat# ALS16654
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