ZC3HC1 / NIPA Antibody (aa320-369)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, E |
---|---|
Primary Accession | Q86WB0 |
Other Accession | 51530 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 55262 Da |
Dilution | ELISA (1:40000), IF (1:100 - 1:500), IHC-P (20 µg/ml), WB (1:500 - 1:1000), |
Gene ID | 51530 |
---|---|
Other Names | ZC3HC1, HSPC216, HNIPA, Zinc finger, C3HC type 1, NIPA |
Target/Specificity | NIPA Antibody detects endogenous levels of total NIPA protein. |
Reconstitution & Storage | PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol. Store at -20°C for up to one year. |
Precautions | ZC3HC1 / NIPA Antibody (aa320-369) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | ZC3HC1 |
---|---|
Synonyms | NIPA |
Function | Essential component of a SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events. |
Cellular Location | Nucleus. |
Tissue Location | Widely expressed. Highly expressed in heart, skeletal muscle and testis. Expressed in brain, placenta, lung, kidney, liver, pancreas, spleen, thymus, prostate, ovary small intestine and colon. Weakly or not expressed in leukocytes |
Volume | 50 µl |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events.
References
Ouyang T.,et al.J. Biol. Chem. 278:30028-30036(2003).
Zhang Q.-H.,et al.Genome Res. 10:1546-1560(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 424:157-164(2003).
Bassermann F.,et al.Cell 122:45-57(2005).
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.