|Application ||WB, IHC-P, IF, Flo|
|Reactivity||Human, Rat, Dog|
|Calculated MW||43482 Da|
|Dilution||Flo (1:100), IF (1:100), IHC-P (10 µg/ml), WB (1:2000),|
|Other Names||HIBCH, HIB-CoA hydrolase, HIBYL-CoA-H, HIBYLCOAH|
|Reconstitution & Storage||PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide. Store at -20°C. Minimize freezing and thawing.|
|Precautions||HIBCH Antibody (clone 5C5) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA.|
|Tissue Location||Highly expressed in liver and kidney, also detected in heart, muscle and brain (at protein level). Not detected in lung.|
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Provided below are standard protocols that you may find useful for product applications.
Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA.
Hawes J.W.,et al.J. Biol. Chem. 271:26430-26434(1996).
Suzuki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
Hillier L.W.,et al.Nature 434:724-731(2005).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Choudhary C.,et al.Science 325:834-840(2009).
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