|Application ||WB, IHC-P, ICC|
|Calculated MW||47269 Da|
|Dilution||ICC (1:2000 - 1:5000), IHC-P (1:500), WB (1:5000 - 1:10000),|
|Other Names||ENO2, Enolase 2, Enolase 2 (gamma, neuronal), Neurone-specific enolase, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE|
|Target/Specificity||These synthetic peptides corresponded to different regions of the NSE-2 gene product, but are shared between the human (NP_001966, NCBI) and rat (AAA41119, NCBI) sequences.|
|Reconstitution & Storage||Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS,” pH 7.2) with sodium azide (0.02%, w/v) added as a preservative. May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 12 months. Sensitive to light.|
|Precautions||ENO2 / NSE Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).|
|Cellular Location||Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.|
|Tissue Location||The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons|
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Provided below are standard protocols that you may find useful for product applications.
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
McAleese S.M.,et al.Eur. J. Biochem. 178:413-417(1988).
van Obberghen E.,et al.J. Neurosci. Res. 19:450-456(1988).
Oliva D.,et al.Gene 79:355-360(1989).
Oliva D.,et al.Genomics 10:157-165(1991).
Ansari-Lari M.A.,et al.Genome Res. 7:268-280(1997).
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