SIDT2 Antibody (N-Terminus)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
---|---|
Primary Accession | Q8NBJ9 |
Other Accession | 51092 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 94454 Da |
Dilution | IHC-P (10 µg/ml), WB (0.5 - 1 µg/ml), |
Gene ID | 51092 |
---|---|
Other Names | SIDT2, CGI-40 |
Target/Specificity | Multiple isoforms of SIDT2 are known to exist; this antibody is predicted to not cross-react with other members of the SID1 transmembrane family. |
Reconstitution & Storage | PBS, 0.02% sodium azide. Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles. |
Precautions | SIDT2 Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SIDT2 |
---|---|
Function | Mediates the translocation of RNA and DNA across the lysosomal membrane during RNA and DNA autophagy (RDA), a process in which RNA or DNA is directly imported into lysosomes in an ATP- dependent manner, and degraded (PubMed:27046251, PubMed:27846365). Involved in the uptake of single-stranded oligonucleotides by living cells, a process called gymnosis (PubMed:28277980). In vitro, mediates the uptake of linear DNA more efficiently than that of circular DNA, but exhibits similar uptake efficacy toward RNA and DNA. Binds long double-stranded RNA (dsRNA) (500 - 700 base pairs), but not dsRNA shorter than 100 bp (By similarity). |
Cellular Location | Lysosome membrane; Multi-pass membrane protein. Cell membrane. Note=Mainly localizes to lysosomes and only partly to the plasma membrane (PubMed:28277980). Lysosomal localization is required for SIDT2-mediated intracellular degradation of endogenous RNA (By similarity). {ECO:0000250|UniProtKB:Q8CIF6, ECO:0000269|PubMed:28277980} |
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Provided below are standard protocols that you may find useful for product applications.
References
Lai C.-H.,et al.Genome Res. 10:703-713(2000).
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Zhang Z.,et al.Protein Sci. 13:2819-2824(2004).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Otsuki T.,et al.DNA Res. 12:117-126(2005).
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