PTRF / CAVIN Antibody (C-Terminus)
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, E |
---|---|
Primary Accession | Q6NZI2 |
Other Accession | 284119 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 43476 Da |
Dilution | IF (20 µg/ml), IHC-P (10 µg/ml), WB (0.5 - 1 µg/ml), |
Gene ID | 284119 |
---|---|
Other Names | PTRF, Cavin-1, CAVIN1, CAVIN, CGL4, FKSG13, TTF-I interacting peptide 12 |
Target/Specificity | At least three isoforms of PTRF are known to exist; this antibody detects all three isoforms. It is predicted to not cross-react with other members of the cavin family. |
Reconstitution & Storage | PBS, 0.02% sodium azide. Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles. |
Precautions | PTRF / CAVIN Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CAVIN1 (HGNC:9688) |
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Synonyms | PTRF |
Function | Plays an important role in caveolae formation and organization. Essential for the formation of caveolae in all tissues (PubMed:18056712, PubMed:18191225, PubMed:19726876). Core component of the CAVIN complex which is essential for recruitment of the complex to the caveolae in presence of calveolin-1 (CAV1). Essential for normal oligomerization of CAV1. Promotes ribosomal transcriptional activity in response to metabolic challenges in the adipocytes and plays an important role in the formation of the ribosomal transcriptional loop. Dissociates transcription complexes paused by DNA-bound TTF1, thereby releasing both RNA polymerase I and pre-RNA from the template (By similarity) (PubMed:18056712, PubMed:18191225, PubMed:19726876). The caveolae biogenesis pathway is required for the secretion of proteins such as GASK1A (By similarity). |
Cellular Location | Membrane, caveola. Cell membrane. Microsome. Endoplasmic reticulum {ECO:0000250|UniProtKB:P85125}. Cytoplasm, cytosol. Mitochondrion. Nucleus Note=Translocates to the cytoplasm from the caveolae upon insulin stimulation (PubMed:17026959). Colocalizes with CAV1 in lipid rafts in adipocytes. Localizes in the caveolae in a caveolin-dependent manner (By similarity). {ECO:0000250|UniProtKB:O54724, ECO:0000269|PubMed:17026959} |
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Background
Plays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex.
References
Wang Y.-G.,et al.Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Jansa P.,et al.EMBO J. 17:2855-2864(1998).
Aboulaich N.,et al.Biochem. J. 383:237-248(2004).
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