|Application ||WB, IHC-P, IF, E|
|Calculated MW||43476 Da|
|Dilution||IF (20 µg/ml), IHC-P (10 µg/ml), WB (0.5 - 1 µg/ml),|
|Other Names||PTRF, Cavin-1, CAVIN1, CAVIN, CGL4, FKSG13, TTF-I interacting peptide 12|
|Target/Specificity||At least three isoforms of PTRF are known to exist; this antibody detects all three isoforms. It is predicted to not cross-react with other members of the cavin family.|
|Reconstitution & Storage||PBS, 0.02% sodium azide. Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||PTRF / CAVIN Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex.|
|Cellular Location||Membrane, caveola. Cell membrane Microsome. Endoplasmic reticulum. Cytoplasm, cytosol. Mitochondrion. Nucleus Note=Found at the surface of the caveolae. Also found in the plasma membrane, microsomal and cytosolic fractions and at a low level in the mitochondrial and nuclear fractions. Translocates to the cytoplasm from the caveolae upon insulin stimulation. CAV1 is necessary to recruit it to the cell membrane (By similarity). Co- localizes with CAV1 in lipid rafts in adipocytes.|
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Plays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex.
Wang Y.-G.,et al.Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Jansa P.,et al.EMBO J. 17:2855-2864(1998).
Aboulaich N.,et al.Biochem. J. 383:237-248(2004).
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