|Application ||WB, IHC-P, IF, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||35423 Da|
|Dilution||IF (20 µg/ml), IHC-P (10 µg/ml), WB (1 - 2 µg/ml),|
|Other Names||SPP1, BSPI, BNSP, Bone sialoprotein 1, ETA-1, Nephropontin, OPN, Secreted phosphoprotein 1, SPP-1, SPP1/CALPHA1 fusion, Urinary stone protein, Uropontin, Osteopontin, Bone sialoprotein I|
|Target/Specificity||SPP1 antibody is human, mouse and rat reactive. Multiple isoforms of SPP1 are known to exist.|
|Reconstitution & Storage||PBS, 0.02% sodium azide. Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||SPP1 / Osteopontin Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.|
|Tissue Location||Bone. Found in plasma.|
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Provided below are standard protocols that you may find useful for product applications.
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Kiefer M.C.,et al.Nucleic Acids Res. 17:3306-3306(1989).
Young M.F.,et al.Genomics 7:491-502(1990).
Shiraga H.,et al.Proc. Natl. Acad. Sci. U.S.A. 89:426-430(1992).
Crosby A.H.,et al.Genomics 27:155-160(1995).
Hijiya N.,et al.Biochem. J. 303:255-262(1994).
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