|Application ||WB, IHC-P, IF, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||123799 Da|
|Dilution||IF (20 µg/ml), IHC-P (10 µg/ml), WB (1 - 2 µg/ml),|
|Other Names||VCL, CMD1W, CMH15, Metavinculin, Vinculin, MVCL|
|Target/Specificity||Vinculin antibody is human, mouse and rat specific. At least three isoforms of Vinculin are known to exist.|
|Reconstitution & Storage||PBS, 0.02% sodium azide. Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||VCL / Vinculin Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.|
|Cellular Location||Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, focal adhesion. Note=Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II- dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions (By similarity) Colocalizes with LIMD1 in the focal adhesions.|
|Tissue Location||Metavinculin is muscle-specific.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Weller P.A.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:5667-5671(1990).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Deloukas P.,et al.Nature 429:375-381(2004).
Moiseyeva E.P.,et al.J. Biol. Chem. 268:4318-4325(1993).
Lubec G.,et al.Submitted (DEC-2008) to UniProtKB.
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