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HSD11B1 / HSD11B Antibody (aa232-292)

Rabbit Polyclonal Antibody

     
  • IHC - HSD11B1 / HSD11B Antibody (aa232-292) ALS16842
    Anti-HSD11B1 / HSD11B antibody IHC staining of human adrenal.
    detail
  • IHC - HSD11B1 / HSD11B Antibody (aa232-292) ALS16842
    Anti-HSD11B1 / HSD11B antibody IHC staining of human liver.
    detail
  • ICC - HSD11B1 / HSD11B Antibody (aa232-292) ALS16842
    Immunofluorescence of paraformaldehyde-fixed HeLa using HSD11B1 antibody at 1:200 dilution.
    detail
  • WB - HSD11B1 / HSD11B Antibody (aa232-292) ALS16842
    Sample (30 ug of whole cell lysate). A: Hep G2 . 12% SDS PAGE. HSD11B1 antibody diluted at 1:1000.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, ICC, IHC-Fr
Primary Accession P28845
Other Accession 3290
Reactivity Human, Sheep
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 32401 Da
Dilution ICC (1:100 - 1:1000), IHC-P (5 µg/ml), WB (1:500 - 1:3000),
Additional Information
Gene ID 3290
Other Names HSD11B1, 11-beta-HSD1, 11-DH, CORTRD2, HSD11B, HSD11L, SDR26C1, HDL, HSD11
Target/Specificity Human HSD11B1
Reconstitution & Storage PBS, pH 7.0, 40% glycerol, 0.01% Thimerosal. Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
PrecautionsHSD11B1 / HSD11B Antibody (aa232-292) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSD11B1 (HGNC:5208)
Synonyms HSD11, HSD11L, SDR26C1
Function Controls the reversible conversion of biologically active glucocorticoids such as cortisone to cortisol, and 11- dehydrocorticosterone to corticosterone in the presence of NADP(H) (PubMed:10497248, PubMed:12460758, PubMed:14973125, PubMed:15152005, PubMed:15280030, PubMed:17593962, PubMed:21453287, PubMed:27927697, PubMed:30902677). Participates in the corticosteroid receptor-mediated anti-inflammatory response, as well as metabolic and homeostatic processes (PubMed:12414862, PubMed:10497248, PubMed:15152005, PubMed:21453287). Plays a role in the secretion of aqueous humor in the eye, maintaining a normotensive, intraocular environment (PubMed:11481269). Bidirectional in vitro, predominantly functions as a reductase in vivo, thereby increasing the concentration of active glucocorticoids (PubMed:12414862, PubMed:10497248, PubMed:11481269, PubMed:12460758). It has broad substrate specificity, besides glucocorticoids, it accepts other steroid and sterol substrates (PubMed:15095019, PubMed:15152005, PubMed:17593962, PubMed:21453287). Interconverts 7-oxo- and 7-hydroxy-neurosteroids such as 7- oxopregnenolone and 7beta-hydroxypregnenolone, 7- oxodehydroepiandrosterone (3beta-hydroxy-5-androstene-7,17-dione) and 7beta-hydroxydehydroepiandrosterone (3beta,7beta-dihydroxyandrost-5-en- 17-one), among others (PubMed:17593962). Catalyzes the stereo-specific conversion of the major dietary oxysterol, 7-ketocholesterol (7- oxocholesterol), into the more polar 7-beta-hydroxycholesterol metabolite (PubMed:15095019, PubMed:15152005). 7-oxocholesterol is one of the most important oxysterols, it participates in several events such as induction of apoptosis, accumulation in atherosclerotic lesions, lipid peroxidation, and induction of foam cell formation (PubMed:15095019). Mediates the 7-oxo reduction of 7-oxolithocholate mainly to chenodeoxycholate, and to a lesser extent to ursodeoxycholate, both in its free form and when conjugated to glycine or taurine, providing a link between glucocorticoid activation and bile acid metabolism (PubMed:21453287). Catalyzes the synthesis of 7-beta- 25-dihydroxycholesterol from 7-oxo-25-hydroxycholesterol in vitro, which acts as a ligand for the G-protein-coupled receptor (GPCR) Epstein-Barr virus-induced gene 2 (EBI2) and may thereby regulate immune cell migration (PubMed:30902677).
Cellular Location Endoplasmic reticulum membrane; Single-pass type II membrane protein
Tissue Location Widely expressed, highest expression in liver, lower in testis, ovary, lung, foreskin fibroblasts, and much lower in kidney (PubMed:1885595). Expressed in liver (at protein level) (PubMed:21453287). Expressed in the basal cells of the corneal epithelium and in the ciliary nonpigmented epithelium (both at mRNA and at protein level) (PubMed:11481269).
Volume 50 µl
Research Areas
Citations (0)
citation

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Background

Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7- ketocholesterol to 7-beta-hydroxycholesterol (By similarity).

References

Tannin G.M.,et al.J. Biol. Chem. 266:16653-16658(1991).
Draper N.,et al.J. Clin. Endocrinol. Metab. 87:4984-4990(2002).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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Discontinued
Cat# ALS16842
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