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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   VASP Antibody (clone 4D6)   

VASP Antibody (clone 4D6)

Mouse Monoclonal Antibody

     
  • IHC - VASP Antibody (clone 4D6) ALS16848
    Anti-VASP antibody IHC staining of human tonsil.
  • IF - VASP Antibody (clone 4D6) ALS16848
    Anti-VASP mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently...
  • WB - VASP Antibody (clone 4D6) ALS16848
    HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VASP...
  • WB - VASP Antibody (clone 4D6) ALS16848
    Western blot of extracts (35 ug) from 9 different cell lines by using anti-VASP monoclonal antibody.
  • FC - VASP Antibody (clone 4D6) ALS16848
    HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid...
  • FC - VASP Antibody (clone 4D6) ALS16848
    Flow cytometry of Jurkat cells, using anti-VASP antibody (Red), compared to a nonspecific...
  • FC - VASP Antibody (clone 4D6) ALS16848
    Flow cytometry of HeLa cells, using anti-VASP antibody (Red), compared to a nonspecific negative...
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, Flo
Primary Accession P50552
Other Accession 7408
Reactivity Human, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 4D6
Calculated MW 39830 Da
Dilution Flo (1:100), IF (1:100), IHC-P (10 µg/ml), WB (1:500 - 1:2000),
Additional Information
Gene ID 7408
Other Names VASP
Target/Specificity Human VASP
Reconstitution & Storage PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide. Store at -20°C. Minimize freezing and thawing.
PrecautionsVASP Antibody (clone 4D6) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VASP
Function Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin- bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cell junction, tight junction. Cell projection, lamellipodium membrane. Cell projection, filopodium membrane. Note=Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions. In pre-apoptotic cells, colocalizes with MEFV in large specks (pyroptosomes)
Tissue Location Highly expressed in platelets.
Volume 50 µl
Research Areas
Citations (0)

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Background

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin- bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.

References

Haffner C.,et al.EMBO J. 14:19-27(1995).
Laurent V.,et al.J. Cell Biol. 144:1245-1258(1999).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Gevaert K.,et al.Nat. Biotechnol. 21:566-569(2003).

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$ 395.00
Cat# ALS16848
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