|Application ||WB, IHC-P, E|
|Calculated MW||44615 Da|
|Dilution||IHC-P (10 µg/ml),|
|Other Names||PGK1, MIG10, Primer recognition protein 2, PRP 2, PGKA, Phosphoglycerate kinase 1|
|Reconstitution & Storage||PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.|
|Precautions||PGK1/Phosphoglycerate Kinase 1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). May play a role in sperm motility (PubMed:26677959).|
|Tissue Location||Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men|
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Provided below are standard protocols that you may find useful for product applications.
In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
Michelson A.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 80:472-476(1983).
Michelson A.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 82:6965-6969(1985).
Kim J.W.,et al.Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
Shichijo S.,et al.Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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