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>   home   >   Products   >   Primary Antibodies   >   MSH6 Antibody (clone 5B11)   

MSH6 Antibody (clone 5B11)

Mouse Monoclonal Antibody

     
  • IHC - MSH6 Antibody (clone 5B11) ALS16874
    Anti-MSH6 antibody IHC staining of human testis.
  • WB - MSH6 Antibody (clone 5B11) ALS16874
    Western blot using MSH6 monoclonal antibody against human MSH6 (AA: 217-395) recombinant protein.
  • WB - MSH6 Antibody (clone 5B11) ALS16874
    Western blot using MSH6 mouse monoclonal antibody against MCF-7 (1), HEK293 (2), and HCT116 (3)...
  • E - MSH6 Antibody (clone 5B11) ALS16874
    Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P52701
Other Accession 2956
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 5B11
Calculated MW 152786 Da
Dilution ELISA (1:10000), IHC-P (1:50), WB (1:500 - 1:2000),
Additional Information
Gene ID 2956
Other Names MSH6, GTBP, HSAP, GTMBP, MutS (E. coli) homolog 6, HMSH6, Sperm-associated protein, p160, G/T mismatch-binding protein, HNPCC5, MutS homolog 6 (E. coli), MutS-alpha 160 kDa subunit
Target/Specificity Human MSH6
Reconstitution & Storage Ascites, 0.03% sodium azide. Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
PrecautionsMSH6 Antibody (clone 5B11) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MSH6 (HGNC:7329)
Synonyms GTBP
Function Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.
Cellular Location Nucleus. Chromosome. Note=Associates with H3K36me3 via its PWWP domain
Citations (0)

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Background

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.

References

Acharya S.,et al.Proc. Natl. Acad. Sci. U.S.A. 93:13629-13634(1996).
Shiwaku H.O.,et al.DNA Res. 4:359-362(1997).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
Palombo F.,et al.Science 268:1912-1914(1995).

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$ 395.00
Cat# ALS16874
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