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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RPS6KA3 / RSK2 Antibody (clone 4E10)   

RPS6KA3 / RSK2 Antibody (clone 4E10)

Mouse Monoclonal Antibody

     
  • IHC - RPS6KA3 / RSK2 Antibody (clone 4E10) ALS16875
    Anti-RPS6KA3 / RSK2 antibody IHC staining of human heart.
  • IF - RPS6KA3 / RSK2 Antibody (clone 4E10) ALS16875
    Immunofluorescence of HepG2 cells using RSK2 mouse monoclonal antibody (green).
  • WB - RPS6KA3 / RSK2 Antibody (clone 4E10) ALS16875
    Western blot using RSK2 monoclonal antibody against human RSK2 (AA: 1-212) recombinant protein.
  • WB - RPS6KA3 / RSK2 Antibody (clone 4E10) ALS16875
    Western blot using RSK2 mouse monoclonal antibody against HeLa (1), MCF-7 (2), and HepG2 (3)...
  • E - RPS6KA3 / RSK2 Antibody (clone 4E10) ALS16875
    Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, ICC, E, Flo
Primary Accession P51812
Other Accession 6197
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 4E10
Calculated MW 83736 Da
Dilution ELISA (1:10000), Flo (1:200 - 1:400), ICC (1:200 - 1:1000), IF (1:1000), IHC-P (1:50), WB (1:500 - 1:2000),
Additional Information
Gene ID 6197
Other Names RPS6KA3, Coffin-Lowry syndrome, HU-2, ISPK1, MAPKAP kinase 1B, MAPKAPK-1b, MRX19, Ribosomal S6 kinase 2, RSK-2, p90-RSK 3, p90RSK3, RSK, RSK2, S6K-alpha-3, S6K-alpha3, CLS, ISPK-1, MAPKAPK1B, p90-RSK2, Pp90RSK2
Target/Specificity Human RPS6KA3 / RSK2
Reconstitution & Storage Ascites, 0.03% sodium azide. Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
PrecautionsRPS6KA3 / RSK2 Antibody (clone 4E10) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPS6KA3
Synonyms ISPK1, MAPKAPK1B, RSK2
Function Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1 and histone H3 at 'Ser-10', which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR- independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro- apoptotic proteins BAD and DAPK1 and suppressing their pro- apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. In LPS-stimulated dendritic cells, is involved in TLR4-induced macropinocytosis, and in myeloma cells, acts as effector of FGFR3-mediated transformation signaling, after direct phosphorylation at Tyr-529 by FGFR3. Phosphorylates DAPK1.
Cellular Location Nucleus. Cytoplasm
Tissue Location Expressed in many tissues, highest levels in skeletal muscle
Research Areas
Citations (0)

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Background

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1 and histone H3 at 'Ser-10', which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR- independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro- apoptotic proteins BAD and DAPK1 and suppressing their pro- apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. In LPS-stimulated dendritic cells, is involved in TLR4-induced macropinocytosis, and in myeloma cells, acts as effector of FGFR3-mediated transformation signaling, after direct phosphorylation at Tyr-529 by FGFR3. Phosphorylates DAPK1.

References

Bjoerbaek C.,et al.Diabetes 44:90-97(1995).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
Ross M.T.,et al.Nature 434:325-337(2005).
Moller D.E.,et al.Am. J. Physiol. 266:C351-C359(1994).

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$ 395.00
Cat# ALS16875
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