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RUNX3 Antibody (clone 2B3)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, ICC, E, Flo |
|---|---|
| Primary Accession | Q13761 |
| Other Accession | 864 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone Names | 2B3 |
| Calculated MW | 44356 Da |
| Dilution | ELISA (1:10000), Flo (1:200 - 1:400), ICC (1:200 - 1:1000), IHC-P (20 µg/ml), WB (1:500 - 1:2000), |
| Gene ID | 864 |
|---|---|
| Other Names | RUNX3, Acute myeloid leukemia gene 2, CBF-alpha-3, PEA2 alpha C, PEBP2 alpha C, PEBP2-alpha C, PEBP2A3, PEBP2aC, AML2, Oncogene AML-2, CBFA3, PEA2-alpha C, Transcription factor AML2 |
| Target/Specificity | Human RUNX3 |
| Reconstitution & Storage | PBS, 0.05% sodium azide, 0.5% protein stabilizer. Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles. |
| Precautions | RUNX3 Antibody (clone 2B3) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RUNX3 |
|---|---|
| Synonyms | AML2, CBFA3, PEBP2A3 |
| Function | Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'- TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (By similarity). May be involved in the control of cellular proliferation and/or differentiation. In association with ZFHX3, up- regulates CDKN1A promoter activity following TGF-beta stimulation (PubMed:20599712). CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity). |
| Cellular Location | Nucleus {ECO:0000255|PROSITE-ProRule:PRU00399, ECO:0000269|PubMed:20100835, ECO:0000269|PubMed:20599712}. Cytoplasm. Note=The tyrosine phosphorylated form localizes to the cytoplasm. Translocates from the cytoplasm to the nucleus following TGF-beta stimulation |
| Tissue Location | Expressed in gastric cancer tissues (at protein level). |
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Provided below are standard protocols that you may find useful for product applications.
Background
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.
References
Bae S.-C.,et al.Gene 159:245-248(1995).
Levanon D.,et al.Genomics 23:425-432(1994).
Groner Y.,et al.Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
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