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HRAS / H-Ras Antibody (aa111-189)

Rabbit Polyclonal Antibody

     
  • IHC - HRAS / H-Ras Antibody (aa111-189) ALS16909
    Anti-HRAS / H-Ras antibody IHC staining of human small intestine.
    detail
  • ICC - HRAS / H-Ras Antibody (aa111-189) ALS16909
    Immunofluorescence of paraformaldehyde-fixed HeLa using H-Ras antibody at 1:200 dilution.
    detail
  • WB - HRAS / H-Ras Antibody (aa111-189) ALS16909
    Sample (30 ug of whole cell lysate).
    detail
  • WB - HRAS / H-Ras Antibody (aa111-189) ALS16909
    H-Ras antibody detects HRAS protein by Western blot analysis.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, ICC
Primary Accession P01112
Other Accession 3265
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 21298 Da
Dilution ICC (1:100 - 1:1000), IHC-P (7.5 µg/ml), WB (1:500 - 1:3000),
Additional Information
Gene ID 3265
Other Names HRAS, C-H-RAS, C-has/bas p21 protein, C-ras-Ki-2 activated oncogene, C-HA-RAS1, CTLO, H-ras, H-RASIDX, GTP- and GDP-binding peptide B, GTPase HRas, H-Ras-1, HAMSV, HRAS1, K-RAS, Ha-Ras, Ha-Ras1 proto-oncoprotein, Transforming protein p21, N-ras, p21r ...
Target/Specificity Human HRAS
Reconstitution & Storage 0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal. Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
PrecautionsHRAS / H-Ras Antibody (aa111-189) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HRAS
Synonyms HRAS1
Function Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:12740440, PubMed:14500341, PubMed:9020151).
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus. Golgi apparatus membrane; Lipid-anchor. Note=The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus.
Tissue Location Widely expressed..
Volume 50 µl
Research Areas
Citations (0)
citation

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Background

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

References

Capon D.J.,et al.Nature 302:33-37(1983).
Reddy E.P.,et al.Science 220:1061-1063(1983).
Sekiya T.,et al.Proc. Natl. Acad. Sci. U.S.A. 81:4771-4775(1984).
Guil S.,et al.Cancer Res. 63:5178-5187(2003).
Puhl H.L. III,et al.Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.

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Discontinued
Cat# ALS16909
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