|Application ||WB, IHC-P, ICC|
|Calculated MW||21298 Da|
|Dilution||ICC (1:100 - 1:1000), IHC-P (7.5 µg/ml), WB (1:500 - 1:3000),|
|Other Names||HRAS, C-H-RAS, C-has/bas p21 protein, C-ras-Ki-2 activated oncogene, C-HA-RAS1, CTLO, H-ras, H-RASIDX, GTP- and GDP-binding peptide B, GTPase HRas, H-Ras-1, HAMSV, HRAS1, K-RAS, Ha-Ras, Ha-Ras1 proto-oncoprotein, Transforming protein p21, N-ras, p21r ...|
|Reconstitution & Storage||0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal. Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.|
|Precautions||HRAS / H-Ras Antibody (aa111-189) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.|
|Cellular Location||Cell membrane. Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus. Golgi apparatus membrane; Lipid-anchor. Note=The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus.|
|Tissue Location||Widely expressed.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Capon D.J.,et al.Nature 302:33-37(1983).
Reddy E.P.,et al.Science 220:1061-1063(1983).
Sekiya T.,et al.Proc. Natl. Acad. Sci. U.S.A. 81:4771-4775(1984).
Guil S.,et al.Cancer Res. 63:5178-5187(2003).
Puhl H.L. III,et al.Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
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