|Application ||WB, IHC-P|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||109540 Da|
|Dilution||IHC-P (10 µg/ml), WB (1:500 - 1:1000),|
|Other Names||ANPEP, Alanyl aminopeptidase, Aminopeptidase M, APN, Aminopeptidase N, AP-N, AP-M, CD13, CD13 antigen, HAPN, gp150, p150, PEPN, Microsomal aminopeptidase, LAP1|
|Reconstitution & Storage||PBS, pH 7.2, 0.05% sodium azide. Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
|Precautions||ANPEP / CD13 Antibody (aa880-930) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||APN, CD13, PEPN|
|Function||Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization.|
|Cellular Location||Cell membrane; Single-pass type II membrane protein. Note=Also found as a soluble form|
|Tissue Location||Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood- brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
Olsen J.,et al.FEBS Lett. 238:307-314(1988).
Look A.T.,et al.J. Clin. Invest. 83:1299-1307(1989).
Zody M.C.,et al.Nature 440:671-675(2006).
Shapiro L.H.,et al.J. Biol. Chem. 266:11999-12007(1991).
Eiz-Vesper B.,et al.Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.