|Application ||WB, IHC-P, ICC|
|Calculated MW||47169 Da|
|Dilution||ICC (1:100 - 1:1000), IHC-P (1:100), WB (1:500 - 1:3000),|
|Other Names||ENO1, Alpha Enolase, Alpha enolase like 1, ENO1L1, Enolase 1, MBP-1, MBPB1, MPB1, MYC promoter-binding protein 1, NNE, Phosphopyruvate hydratase, Alpha-enolase, Tau-crystallin, MBP1, MPB-1, C-myc promoter-binding protein, Enolase 1, (alpha), Enolase- ...|
|Target/Specificity||Human ENO1 / Alpha Enolase|
|Reconstitution & Storage||0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal. Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.|
|Precautions||ENO1 / Alpha Enolase Antibody (aa199-434) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ENO1L1, MBPB1, MPB1|
|Function||Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.|
|Cellular Location||Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line|
|Tissue Location||The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons|
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Provided below are standard protocols that you may find useful for product applications.
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
Giallongo A.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:6741-6745(1986).
Giallongo A.,et al.Eur. J. Biochem. 190:567-573(1990).
Ray R.,et al.Mol. Cell. Biol. 11:2154-2161(1991).
Walter M.,et al.J. Autoimmun. 8:931-945(1995).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
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