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AKT3 Antibody (clone 9H8)

Mouse Monoclonal Antibody

     
  • IHC - AKT3 Antibody (clone 9H8) ALS16969
    Anti-AKT3 antibody IHC staining of human kidney.
    detail
  • IF - AKT3 Antibody (clone 9H8) ALS16969
    Immunofluorescent staining of HeLa cells using anti-AKT3 mouse monoclonal antibody.
    detail
  • IF - AKT3 Antibody (clone 9H8) ALS16969
    Anti-AKT3 mouse monoclonal antibody immunofluorescent staining of HeLa cells transiently...
    detail
  • WB - AKT3 Antibody (clone 9H8) ALS16969
    HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3...
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF
Primary Accession Q9Y243
Other Accession 10000
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG3
Clone Names 9H8
Calculated MW 55775 Da
Dilution IF (1:100), IHC-P (10 µg/ml), WB (1:500),
Additional Information
Gene ID 10000
Other Names AKT3, AKT3/PKBgamma, PRKBG, PKB gamma, Protein kinase Akt-3, RAC-gamma, MPPH, PKB-GAMMA, PKBG, Protein kinase B gamma, RAC-PK-gamma, STK-2
Target/Specificity Human AKT3
Reconstitution & Storage PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide. Store at -20°C. Minimize freezing and thawing.
PrecautionsAKT3 Antibody (clone 9H8) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AKT3
Synonyms PKBG
Function AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down- regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase- dependent apoptosis.
Cellular Location Nucleus. Cytoplasm. Membrane; Peripheral membrane protein Note=Membrane-associated after cell stimulation leading to its translocation
Tissue Location In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney
Research Areas
Citations (0)
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Background

AKT3 is one of 3 closely related serine/threonine- protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase- dependent apoptosis.

References

Brodbeck D.,et al.J. Biol. Chem. 274:9133-9136(1999).
Nakatani K.,et al.Biochem. Biophys. Res. Commun. 257:906-910(1999).
Masure S.,et al.Eur. J. Biochem. 265:353-360(1999).
Li X.,et al.Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
Wiemann S.,et al.Genome Res. 11:422-435(2001).

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Discontinued
Cat# ALS16969
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