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CHTOP / FOP Antibody (C-Terminus, clone KT64)
Rat Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, ICC, IP |
|---|---|
| Primary Accession | Q9Y3Y2 |
| Other Accession | 26097 |
| Reactivity | Human, Mouse |
| Host | Rat |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone Names | KT64 |
| Calculated MW | 26397 Da |
| Dilution | ICC (1:100), IHC-P (10 µg/ml), WB (1:1000), |
| Gene ID | 26097 |
|---|---|
| Other Names | CHTOP, C1orf77, Chromatin target of PRMT1, DKFZP547E1010, FOP, Friend of PRMT1 protein, Pp7704, SRAG, SRAG-3, SRAG-5, FL-SRAG, RP1-178F15.2 |
| Target/Specificity | Mouse CHTOP / FOP |
| Reconstitution & Storage | PBS, 0.1% sodium azide. Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
| Precautions | CHTOP / FOP Antibody (C-Terminus, clone KT64) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CHTOP |
|---|---|
| Synonyms | C1orf77, FOP |
| Function | Plays an important role in the ligand-dependent activation of estrogen receptor target genes (PubMed:19858291). May play a role in the silencing of fetal globin genes (PubMed:20688955). Recruits the 5FMC complex to ZNF148, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (By similarity). Plays an important role in the tumorigenicity of glioblastoma cells. Binds to 5-hydroxymethylcytosine (5hmC) and associates with the methylosome complex containing PRMT1, PRMT5, MEP50 and ERH. The CHTOP- methylosome complex associated with 5hmC is recruited to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789). |
| Cellular Location | Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus speckle. Note=Mostly associated with facultative heterochromatin (By similarity). Localizes to regions surrounding nuclear speckles known as perispeckles in which TREX complex assembly seems to occur (PubMed:23826332) {ECO:0000250|UniProtKB:Q9CY57, ECO:0000269|PubMed:23826332} |
| Tissue Location | Expressed in an erythroid progenitor cell line derived from peripheral blood. Expressed in glioblastoma cells (PubMed:25284789). |
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Provided below are standard protocols that you may find useful for product applications.
Background
Plays an important role in the ligand-dependent activation of estrogen receptor target genes. May play a role in the silencing of fetal globin genes. Recruits the 5FMC complex to ZNF148, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.
References
Xu X.,et al.Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases.
Wan D.,et al.Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
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