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NCL / Nucleolin Antibody (clone 4E2)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, ICC, Flo |
|---|---|
| Primary Accession | P19338 |
| Other Accession | 4691 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone Names | 4E2 |
| Calculated MW | 76614 Da |
| Dilution | Flo (5 - 10 µg/ml), ICC (1 - 10 µg/ml), IHC-P (10 µg/ml), WB (1 µg/ml), |
| Gene ID | 4691 |
|---|---|
| Other Names | NCL, Nucleolin, C23, Protein C23 |
| Target/Specificity | Human Nucleolin |
| Reconstitution & Storage | PBS, pH 7.2, 50% glycerol. Store at -20°C. |
| Precautions | NCL / Nucleolin Antibody (clone 4E2) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NCL |
|---|---|
| Function | Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG- 3' repeats more tightly than the telomeric single-stranded DNA 5'- TTAGGG-3' repeats. |
| Cellular Location | Nucleus, nucleolus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs |
| Volume | 50 µl |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
References
Srivastava M.,et al.FEBS Lett. 250:99-105(1989).
Srivastava M.,et al.J. Biol. Chem. 265:14922-14931(1990).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
Krantz S.,et al.Biochim. Biophys. Acta 1266:109-112(1995).
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