|Application ||IHC-P, IF, E|
|Calculated MW||121520 Da|
|Dilution||ELISA (1:10000), IF (1:100 - 1:500), IHC-P (10 µg/ml),|
|Other Names||UBN1, HIRA-binding protein, Protein VT4, VT, Ubinuclein-1, VT4, Ubinuclein 1|
|Target/Specificity||Ubinuclein Antibody detects endogenous levels of total Ubinuclein protein.|
|Reconstitution & Storage||PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol. Store at -20°C.|
|Precautions||UBN1 / Ubinuclein 1 Antibody (aa161-210) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a novel regulator of senescence. Involved in the formation of senescence-associated heterochromatin foci (SAHF), which represses expression of proliferation-promoting genes. Binds to proliferation-promoting genes. May be required for replication- independent chromatin assembly.|
|Cellular Location||Nucleus, nucleoplasm. Nucleus, PML body. Cell junction, tight junction. Note=Localized as a nuclear speckled-like pattern in proliferating primary fibroblasts Colocalizes with HIRA, PML and SP100 in PML bodies of senescent cells. Colocalizes with TJP1 and CLDN1. Detected along the upper granular cell layer of epidermis. When overexpressed, accumulates in the nucleus in cells showing defective cytokinesis|
|Tissue Location||Ubiquitous. Also expressed in numerous tumors and cancer cell lines.|
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Acts as a novel regulator of senescence. Involved in the formation of senescence-associated heterochromatin foci (SAHF), which represses expression of proliferation-promoting genes. Binds to proliferation-promoting genes. May be required for replication- independent chromatin assembly.
Aho S.,et al.J. Cell Biol. 148:1165-1176(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Martin J.,et al.Nature 432:988-994(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Tesmer V.,et al.Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases.
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