|Application ||WB, IHC-P|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||46281 Da|
|Dilution||IHC-P (2.5 µg/ml), WB (1:1000),|
|Other Names||CASP9, APAF3, CASPASE-9c, ICE-LAP6, MCH6, ICE-like apoptotic protease 6, APAF-3, Apoptotic protease Mch-6, CASP-9, Caspase 9, Caspase-9, PPP1R56|
|Target/Specificity||This Polyclonal antibody is directed against human Caspase-9 protein. The product was purified from serum by protein A chromatography.|
|Reconstitution & Storage||PBS, pH 7.4, 0.02% sodium azide. Store at -20°C for up to one year.|
|Precautions||CASP9 / Caspase 9 Antibody (aa299-318) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP- ribose) polymerase (PARP).|
|Tissue Location||Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP- ribose) polymerase (PARP).
Duan H.,et al.J. Biol. Chem. 271:16720-16724(1996).
Srinivasula S.M.,et al.J. Biol. Chem. 271:27099-27106(1996).
Hadano S.,et al.Mamm. Genome 10:757-760(1999).
Srinivasula S.M.,et al.Cancer Res. 59:999-1002(1999).
Izawa M.,et al.Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.