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HHEX / HEX Antibody (clone 3F4)

Mouse Monoclonal Antibody

     
  • IHC - HHEX / HEX Antibody (clone 3F4) ALS17006
    Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
    detail
  • IF - HHEX / HEX Antibody (clone 3F4) ALS17006
    Anti-HHex mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently...
    detail
  • IF - HHEX / HEX Antibody (clone 3F4) ALS17006
    Immunofluorescent staining of HT29 cells using anti-HHex mouse monoclonal antibody.
    detail
  • IF - HHEX / HEX Antibody (clone 3F4) ALS17006
    Immunofluorescent staining of HeLa cells using anti-HHex mouse monoclonal antibody.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IF
Primary Accession Q03014
Other Accession 3087
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Clone Names 3F4
Calculated MW 30022 Da
Dilution IF (1:100), IHC-P (2.5 µg/ml),
Additional Information
Gene ID 3087
Other Names HHEX, HEX, HMPH, Homeobox protein HEX, HOX11L-PEN, PRHX, Homeobox protein PRH, PRH
Target/Specificity Human HHEX / HEX
Reconstitution & Storage PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide. Store at -20°C. Minimize freezing and thawing.
PrecautionsHHEX / HEX Antibody (clone 3F4) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HHEX
Synonyms HEX, PRH, PRHX
Function Recognizes the DNA sequence 5'-ATTAA-3' (By similarity). Transcriptional repressor (By similarity). Activator of WNT-mediated transcription in conjunction with CTNNB1 (PubMed:20028982). Establishes anterior identity at two levels; acts early to enhance canonical WNT- signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL (By similarity). Inhibits EIF4E-mediated mRNA nuclear export (PubMed:12554669). May play a role in hematopoietic differentiation (PubMed:8096636).
Cellular Location Nucleus {ECO:0000250|UniProtKB:P43120}. Nucleus, nuclear body. Cytoplasm
Tissue Location Liver and promyelocytic leukemia cell line HL-60.
Research Areas
Citations (0)
citation

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Background

Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL (By similarity).

References

Crompton M.R.,et al.Nucleic Acids Res. 20:5661-5667(1992).
Hromas R.A.,et al.Biochem. Biophys. Res. Commun. 195:976-983(1993).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 429:375-381(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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$ 467.50
Cat# ALS17006
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